1 下载ffmeg

wget https://github.com/BtbN/FFmpeg-Builds/releases/download/latest/ffmpeg-master-latest-win64-gpl.zip

把bin目录配置到path中，或者cd到ffmeg的bin目录下调用脚本

2 下载pydub

pip install -i https://pypi.tuna.tsinghua.edu.cn/simple pydub

3 使用

# 导入
from pydub import AudioSegment
song = AudioSegment.from_wav("G:/click2.wav")
# 剪辑前一秒 1000=1s
one_s = 1000
one_s_song = song[:one_s]
# 导出
one_s_song.export("G:/click3.wav", format="wav")
<_io.BufferedRandom name='G:/click3.wav'>

4 教程

https://github.com/jiaaro/pydub