历史分析：
- pathway是一个不得不研究的主体，比如我们的paper就给很多GO pathway的基因打分了，很简单就是一个求均值。
- 关于complex的活性打分，就得使用几何平均，因为缺失任何一个都会导致complex失活。
现有工具：
- AUCell allows to identify cells with active gene sets (e.g. signatures, gene modules...) in single-cell RNA-seq data. AUCell uses the "Area Under the Curve" (AUC) to calculate whether a critical subset of the input gene set is enriched within the expressed genes for each cell.
- 为基因集进行打分常见的富集分析软件GSVA
- Calculate module scores for feature expression programs in single cells：AddModuleScore
目前任务：给Wnt和RA通路打分
初步设想：分component activity和target gene activity两个维度来衡量。
参考文献：wnt signaling target genes
- Wnt target genes and where to find them
- Wnt Target genes
- Multiple Targets of the Canonical WNT/β-Catenin Signaling in Cancers
- Wnt/β-Catenin Target Genes in Colon Cancer Metastasis: The Special Case of L1CAM
- Comprehensive analysis of β-catenin target genes in colorectal carcinoma cell lines with deregulated Wnt/β-catenin signaling
待续~
参考：
标签：target,genes,pathway,Wnt,signalling,gene,打分 From： https://www.cnblogs.com/leezx/p/16600530.html