使用snpeff软件构建本地注释库

001、软件下载

官网：https://pcingola.github.io/SnpEff/

下载，然后解压：

(base) [root@pc1 software]# wget -c https://snpeff.blob.core.windows.net/versions/snpEff_latest_core.zip          ## 下载
--2023-10-06 05:03:27--  https://snpeff.blob.core.windows.net/versions/snpEff_latest_core.zip
Resolving snpeff.blob.core.windows.net (snpeff.blob.core.windows.net)... 52.239.234.228
Connecting to snpeff.blob.core.windows.net (snpeff.blob.core.windows.net)|52.239.234.228|:443... connected.
HTTP request sent, awaiting response... 200 OK
Length: 66465201 (63M) [application/zip]
Saving to: ‘snpEff_latest_core.zip’

100%[===========================================================================================================>] 66,465,201   315KB/s   in 1m 43s

2023-10-06 05:05:12 (629 KB/s) - ‘snpEff_latest_core.zip’ saved [66465201/66465201]

(base) [root@pc1 software]# ls
snpEff_latest_core.zip
(base) [root@pc1 software]# unzip snpEff_latest_core.zip &> /dev/zero                                        ## 解压
(base) [root@pc1 software]# ls
snpEff  snpEff_latest_core.zip
(base) [root@pc1 software]# cd snpEff/                                                                       ## 查看软件内容
(base) [root@pc1 snpEff]# ls 
examples  exec  galaxy  LICENSE.md  scripts  snpEff.config  snpEff.jar  SnpSift.jar

002、java环境

snpeff软件对java的版本有一定要求，centos7默认的java1.8不行，因此安装自带的java11

003、构建注释库，需要参考基因组xxx.fa和基因组注释文件xxx.gff

004、构建结果

参考：

01、

02、