标签：-- fastq barcode gz split file fqkit
双端测序数据barcode拆分工具
- 目前只支持单barcode拆分
- 支持gz压缩文件输入输入
- 参数支持barcode碱基容错
- 支持一个样本对应多个barcode
repo :
github : https://github.com/sharkLoc/fqkit.git
install :
cargo install fqkit
# or
git clone https://github.com/sharkLoc/fqkit.git
cd fqkit
cargo b --release
# mv target/release/fqkit to anywhere you want
usage :
fqkit barcode --help
split barcode for PE reads
Usage: fqkit barcode [OPTIONS] --read1 <READ1> --read2 <READ2> --barcode <BAR>
Options:
-1, --read1 <READ1> input read1 fastq[.gz] file
-2, --read2 <READ2> input read2 fastq[.gz] file, barcode in this file
-b, --barcode <BAR> barcode list file, format eg:
ATGCAGTG sample1
TGCAGTAC sample2
-m, --mode <MODE> barcode position mode, 1:left, 2:right [default: 2]
-r, --rev_comp barcode reverse complement
-e, --error <MISMATCH> barcode mismatch base count [default: 0]
-o, --outdir <OUTDIR> fastq file output dir [default: .]
-h, --help Print help information
标签：--,
fastq,
barcode,
gz,
split,
file,
fqkit
From： https://www.cnblogs.com/mmtinfo/p/17031678.html