001、sra数据转换fastq数据报错如下:
002、报错产生的原因“:
没有网络连接。
003、验证
a、无网络连接
[root@PC1 test1]# ls SRR11076280 [root@PC1 test1]# ping -c 3 www.baidu.com ## 检测网络连通性 ping: www.baidu.com: Name or service not known [root@PC1 test1]# fastq-dump --split-files SRR11076280 ## 将sra数据转换为fastq 2024-01-18T12:49:09 fastq-dump.3.0.10 warn: directory not found while opening manager within virtual file system module - can't open CM004562.1 as a RefSeq or as a WGS 2024-01-18T12:49:09 fastq-dump.3.0.10 err: directory not found while opening manager within virtual file system module - failed SRR11076280 ============================================================= An error occurred during processing. A report was generated into the file '/root/ncbi_error_report.txt'. If the problem persists, you may consider sending the file to '[email protected]' for assistance. ============================================================= fastq-dump quit with error code 3
b、有网络连接
[root@PC1 test1]# ls SRR11076280 [root@PC1 test1]# ping -c 3 www.baidu.com ## 网络连通性 PING www.a.shifen.com (110.242.68.3) 56(84) bytes of data. 64 bytes from 110.242.68.3 (110.242.68.3): icmp_seq=1 ttl=128 time=13.8 ms 64 bytes from 110.242.68.3 (110.242.68.3): icmp_seq=2 ttl=128 time=17.4 ms 64 bytes from 110.242.68.3 (110.242.68.3): icmp_seq=3 ttl=128 time=15.6 ms --- www.a.shifen.com ping statistics --- 3 packets transmitted, 3 received, 0% packet loss, time 2003ms rtt min/avg/max/mdev = 13.843/15.672/17.495/1.490 ms [root@PC1 test1]# fastq-dump --split-files SRR11076280 ## 转换为fastq,可以正确转换 ^C[root@PC1 test1]# ls SRR11076280 SRR11076280_1.fastq SRR11076280_2.fastq [root@PC1 test1]# ll -h total 6.3G -rw-r--r--. 1 root root 6.2G Jan 18 20:40 SRR11076280 -rw-r--r--. 1 root root 39M Jan 18 20:53 SRR11076280_1.fastq -rw-r--r--. 1 root root 39M Jan 18 20:53 SRR11076280_2.fastq
。
标签:test1,quit,code,fastq,PC1,SRR11076280,110.242,root From: https://www.cnblogs.com/liujiaxin2018/p/17972270